5ZOF
Crystal Structure of D181A/R192F hFen1 in complex with DNA
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Flap endonuclease 1 | polymer | 333 | 37613.3 | 1 | UniProt (P39748) Pfam (PF00752) Pfam (PF00867) In PDB | Homo sapiens (Human) | FEN-1,DNase IV,Flap structure-specific endonuclease 1,Maturation factor 1,hFEN-1 |
2 | B | DNA (5'-D(*CP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*G)-3') | polymer | 18 | 5477.5 | 1 | synthetic construct | ||
3 | C | DNA (5'-D(*GP*CP*CP*CP*GP*TP*CP*C)-3') | polymer | 8 | 2363.6 | 1 | synthetic construct | ||
4 | D | DNA (5'-D(*AP*CP*TP*TP*TP*GP*AP*GP*GP*CP*AP*GP*AP*G)-3') | polymer | 14 | 4344.8 | 1 | synthetic construct | ||
5 | A | POTASSIUM ION | non-polymer | 39.1 | 1 | Chemie (K) | |||
6 | water | water | 18.0 | 10 | Chemie (HOH) |
Sequence modifications
A: 1 - 333 (UniProt: P39748)
PDB | External Database | Details |
---|---|---|
Ala 181 | Asp 181 | engineered mutation |
Phe 192 | Arg 192 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 49799.2 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 39.1 | |
All* | Total formula weight | 49838.3 |