5ZEC
Crystal structure of Kluyveromyces polyspora ADH (KpADH) mutant (Q136N/F161V/S196G/E214G/S237C)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Uncharacterized protein ADH | polymer | 342 | 38801.1 | 2 | UniProt (A7TM80) Pfam (PF01370) In PDB | Vanderwaltozyma polyspora DSM 70294 (Kluyveromyces polysporus) | |
| 2 | A, B | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 2 | Chemie (NAP) | |||
| 3 | A, B | 1,2-ETHANEDIOL | non-polymer | 62.1 | 6 | Chemie (EDO) | |||
| 4 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 5 | A | ISOPROPYL ALCOHOL | non-polymer | 60.1 | 1 | Chemie (IPA) | |||
| 6 | B | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 7 | B | ETHANOL | non-polymer | 46.1 | 1 | Chemie (EOH) | |||
| 8 | water | water | 18.0 | 779 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 342 (UniProt: A7TM80)
| PDB | External Database | Details |
|---|---|---|
| Asn 136 | Gln 136 | engineered mutation |
| Val 161 | Phe 161 | engineered mutation |
| Gly 196 | Ser 196 | engineered mutation |
| Gly 214 | Glu 214 | engineered mutation |
| Cys 237 | Ser 237 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 77602.1 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 2163.6 | |
| All* | Total formula weight | 79765.7 |
