5YZX

Crystal structure of E.coli LysU T146D mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Lysine--tRNA ligase, heat inducible	polymer	525	60083.7	3	UniProt (P0A8N5) Pfam (PF01336) Pfam (PF00152)	Escherichia coli (strain K12)	Lysyl-tRNA synthetase,LysRS
2	D, H, L (A, B, C)	BIS(ADENOSINE)-5'-TETRAPHOSPHATE	non-polymer		836.4	3	Chemie (B4P)
3	E, F, G, I, J... (A, B, C)	CALCIUM ION	non-polymer		40.1	9	Chemie (CA)

Sequence modifications

A, B, C: 0 - 504 (UniProt: P0A8N5)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Asp 146	Thr 147	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	180251.2
Non-Polymers*	Number of molecules	12
	Total formula weight	2869.9
All*	Total formula weight	183121.1

*Water molecules are not included.