5YZX
Crystal structure of E.coli LysU T146D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 144.544, 249.855, 179.652 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.147 - 3.200 |
| R-factor | 0.2376 |
| Rwork | 0.236 |
| R-free | 0.26850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e22 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.626 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 6.880 | 3.200 |
| Rmerge | 0.220 | 0.094 | 0.586 |
| Rmeas | 0.246 | 0.103 | 0.676 |
| Rpim | 0.108 | 0.043 | 0.330 |
| Total number of observations | 278081 | ||
| Number of reflections | 56309 | 5875 | 5540 |
| <I/σ(I)> | 5.4 | ||
| Completeness [%] | 99.5 | 99.5 | 98.9 |
| Redundancy | 4.9 | 5.7 | 3.9 |
| CC(1/2) | 0.994 | 0.711 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M NH4SO4, 0.3M Na Formate, 0.1 M Na Cacodylate pH 6.5, 3% PGA, and 30% PEG400, |
