5YO1
Structure of ePepN E298A mutant in complex with Puromycin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Aminopeptidase N | polymer | 891 | 101383.2 | 1 | UniProt (P04825) Pfam (PF17900) Pfam (PF01433) Pfam (PF11940) Pfam (PF17432) | Escherichia coli K12 | Alpha-aminoacylpeptide hydrolase, E. coli Amino peptidase N |
| 2 | B (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 3 | C, D (A) | PUROMYCIN | non-polymer | 471.5 | 2 | Chemie (PUY) | |||
| 4 | E (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 5 | F, G, H, I, J... (A) | GLYCEROL | non-polymer | 92.1 | 12 | Chemie (GOL) | |||
| 6 | R (A) | water | water | 18.0 | 987 | Chemie (HOH) |
Sequence modifications
A: 1 - 870 (UniProt: P04825)
| PDB | External Database | Details |
|---|---|---|
| Met -20 | - | initiating methionine |
| Gly -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Ser -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 298 | Glu 298 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 101383.2 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 2136.5 | |
| All* | Total formula weight | 103519.7 |
