5YO1

Structure of ePepN E298A mutant in complex with Puromycin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Aminopeptidase N	polymer	891	101383.2	1	UniProt (P04825) Pfam (PF17900) Pfam (PF01433) Pfam (PF11940) Pfam (PF17432) In PDB	Escherichia coli K12	Alpha-aminoacylpeptide hydrolase, E. coli Amino peptidase N
2	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	A	PUROMYCIN	non-polymer		471.5	2	Chemie (PUY)
4	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	A	GLYCEROL	non-polymer		92.1	12	Chemie (GOL)
6		water	water		18.0	987	Chemie (HOH)

Sequence modifications

A: 1 - 870 (UniProt: P04825)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Ala 298	Glu 298	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	101383.2
Non-Polymers*	Number of molecules	16
	Total formula weight	2136.5
All*	Total formula weight	103519.7

*Water molecules are not included.