5YO1
Structure of ePepN E298A mutant in complex with Puromycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-04-20 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.325, 120.325, 170.012 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.900 - 2.500 |
| R-factor | 0.12876 |
| Rwork | 0.126 |
| R-free | 0.18427 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hpo |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.854 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.900 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 49464 |
| <I/σ(I)> | 2.13 |
| Completeness [%] | 99.0 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2.0M Sodium Malonate |
