5XIT

Crystal structure of RNF168 UDM1 in complex with Lys63-linked diubiquitin, form II

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (D, F)	Ubiquitin-40S ribosomal protein S27a	polymer	76	8604.8	2	UniProt (P62983) Pfam (PF00240)	Mus musculus (Mouse)	Ubiquitin carboxyl extension protein 80
2	B, D (E, A)	E3 ubiquitin-protein ligase RNF168	polymer	81	9618.5	2	UniProt (Q8IYW5)	Homo sapiens (Human)	hRNF168,RING finger protein 168,RING-type E3 ubiquitin transferase RNF168
3	C, E (H, B)	Ubiquitin-40S ribosomal protein S27a	polymer	77	8691.9	2	UniProt (P62983) Pfam (PF00240)	Mus musculus (Mouse)	Ubiquitin carboxyl extension protein 80
4	G, K, L, M, N (D, E, A)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
5	H, I, J (E)	PRASEODYMIUM ION	non-polymer		140.9	3	Chemie (PR)
6	O, P, Q, R, S... (D, E, H, A, B...)	water	water		18.0	58	Chemie (HOH)

Sequence modifications

D, F: 1 - 76 (UniProt: P62983)

PDB	External Database	Details
Arg 63	Lys 63	engineered mutation

E, A: 113 - 188 (UniProt: Q8IYW5)

PDB	External Database	Details
Gly 108	-	expression tag
Pro 109	-	expression tag
Gly 110	-	expression tag
His 111	-	expression tag
Met 112	-	expression tag

H, B: 1 - 76 (UniProt: P62983)

PDB	External Database	Details
Asp 77	-	see sequence details

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	53830.5
Non-Polymers*	Number of molecules	8
	Total formula weight	883.2
All*	Total formula weight	54713.7

*Water molecules are not included.