5XIT
Crystal structure of RNF168 UDM1 in complex with Lys63-linked diubiquitin, form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2015-10-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 |
Unit cell lengths | 45.372, 50.019, 64.407 |
Unit cell angles | 73.49, 69.69, 73.82 |
Refinement procedure
Resolution | 46.986 - 2.250 |
R-factor | 0.2213 |
Rwork | 0.220 |
R-free | 0.23910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fid |
RMSD bond length | 0.003 |
RMSD bond angle | 0.615 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 21397 | |
<I/σ(I)> | 20.2 | 1.8 |
Completeness [%] | 90.2 | 91.5 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 23% PEG MME 2000, 0.1 M Bis-Tris (pH 6.5), 10 mM Pr acetate |