5X7X

The crystal structure of the nucleosome containing H3.3 at 2.18 angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3.3	polymer	139	15643.3	2	UniProt (P84243) Pfam (PF00125) In PDB	Homo sapiens (Human)
2	B, F	Histone H4	polymer	106	11676.7	2	UniProt (P62805) Pfam (PF15511) In PDB	Homo sapiens (Human)
3	C, G	Histone H2A type 1-B/E	polymer	133	14447.8	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211) In PDB	Homo sapiens (Human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H	Histone H2B type 1-J	polymer	129	14217.5	2	UniProt (P06899) Pfam (PF00125) In PDB	Homo sapiens (Human)	Histone H2B.1,Histone H2B.r,H2B/r
5	I, J	DNA (146-MER)	polymer	146	45053.9	2		Homo sapiens
6	A, C, E, G	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
7	J, E, I	MANGANESE (II) ION	non-polymer		54.9	13	Chemie (MN)
8		water	water		18.0	436	Chemie (HOH)

Sequence modifications

A, E: 0 - 135 (UniProt: P84243)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

B, F: 0 - 102 (UniProt: P62805)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

C, G: 0 - 129 (UniProt: P04908)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

D, H: 0 - 125 (UniProt: P06899)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	202078.3
Non-Polymers*	Number of molecules	17
	Total formula weight	856.0
All*	Total formula weight	202934.3

*Water molecules are not included.