5X7X

The crystal structure of the nucleosome containing H3.3 at 2.18 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-19
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	98.887, 107.509, 167.415
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.600 - 2.184
R-factor	0.2279
Rwork	0.227
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cv5
RMSD bond length	0.006
RMSD bond angle	0.818
Data reduction software	HKL
Data scaling software	HKL-2000 (2.3.10)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.260
High resolution limit [Å]	2.180	2.180
Rmerge	0.081	0.483
Number of reflections	92136
<I/σ(I)>	10.7	3.5
Completeness [%]	99.5	98.9
Redundancy	14.2	8.9
CC(1/2)		0.531

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	potassium cacodylate, potassium chloride, manganese chloride