5X2H
Crystal structure of Campylobacter jejuni Cas9 in complex with sgRNA and target DNA (AGAAACA PAM)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9 | polymer | 835 | 96800.2 | 1 | UniProt (Q0P897) Pfam (PF18541) Pfam (PF22129) Pfam (PF22131) | Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) | |
| 2 | B (B) | sgRNA | polymer | 93 | 30023.8 | 1 | Campylobacter jejuni | ||
| 3 | C (C) | Target DNA strand | polymer | 28 | 8467.5 | 1 | synthetic construct | ||
| 4 | D (D) | Non-target DNA strand | polymer | 8 | 2468.7 | 1 | synthetic construct | ||
| 5 | E (B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 6 | F, G, H, I (A, B, C, D) | water | water | 18.0 | 275 | Chemie (HOH) |
Sequence modifications
A: 1 - 480 (UniProt: Q0P897)
A: 642 - 984 (UniProt: Q0P897)
| PDB | External Database | Details |
|---|---|---|
| Ser -5 | - | expression tag |
| Ser -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 636 | - | linker |
| Gly 637 | - | linker |
| Gly 638 | - | linker |
| Ser 639 | - | linker |
| Gly 640 | - | linker |
| Gly 641 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 137760.1 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 62.1 | |
| All* | Total formula weight | 137822.2 |
