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5X2H

Crystal structure of Campylobacter jejuni Cas9 in complex with sgRNA and target DNA (AGAAACA PAM)

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)
CRISPR-associated endonuclease Cas9polymer83596800.21UniProt (Q0P897)
Pfam (PF18541)
Pfam (PF22129)
Pfam (PF22131)
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
2B
(B)
sgRNApolymer9330023.81Campylobacter jejuni
3C
(C)
Target DNA strandpolymer288467.51synthetic construct
4D
(D)
Non-target DNA strandpolymer82468.71synthetic construct
5E
(B)
1,2-ETHANEDIOLnon-polymer62.11Chemie (EDO)
6F, G, H, I
(A, B, C, D)
waterwater18.0275Chemie (HOH)
Sequence modifications
A: 1 - 480 (UniProt: Q0P897)
PDBExternal DatabaseDetails
Ser -5-expression tag
Ser -4-expression tag
Gly -3-expression tag
Ser -2-expression tag
Gly -1-expression tag
His 0-expression tag
A: 642 - 984 (UniProt: Q0P897)
PDBExternal DatabaseDetails
Gly 636-linker
Gly 637-linker
Gly 638-linker
Ser 639-linker
Gly 640-linker
Gly 641-linker
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains4
Total formula weight137760.1
Non-Polymers*Number of molecules1
Total formula weight62.1
All*Total formula weight137822.2
*Water molecules are not included.

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PDB entries from 2025-10-15

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