5X2H
Crystal structure of Campylobacter jejuni Cas9 in complex with sgRNA and target DNA (AGAAACA PAM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 102.039, 103.911, 134.458 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.805 - 2.300 |
| R-factor | 0.2015 |
| Rwork | 0.200 |
| R-free | 0.23110 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.515 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.910 | |
| High resolution limit [Å] | 2.300 | |
| Rpim | 0.032 | 0.316 |
| Number of reflections | 64066 | |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 10.4 | |
| CC(1/2) | 0.986 | 0.875 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12.0-14.5% PEG 2000, 0.4M ammonium acetate |
