5X2H

Crystal structure of Campylobacter jejuni Cas9 in complex with sgRNA and target DNA (AGAAACA PAM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	102.039, 103.911, 134.458
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.805 - 2.300
R-factor	0.2015
Rwork	0.200
R-free	0.23110
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.515
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	103.910
High resolution limit [Å]	2.300
Rpim	0.032	0.316
Number of reflections	64066
<I/σ(I)>	12.4
Completeness [%]	99.8
Redundancy	10.4
CC(1/2)	0.986	0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	12.0-14.5% PEG 2000, 0.4M ammonium acetate