5X0W

Molecular mechanism for the binding between Sharpin and HOIP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G	E3 ubiquitin-protein ligase RNF31	polymer	166	18828.4	4	UniProt (Q96EP0) Pfam (PF16678) In PDB	Homo sapiens (Human)	HOIL-1-interacting protein,HOIP,RING finger protein 31,Zinc in-between-RING-finger ubiquitin-associated domain protein
2	B, D, F, H	Sharpin	polymer	108	11346.8	4	UniProt (Q9H0F6) In PDB	Homo sapiens (Human)	Shank-associated RH domain-interacting protein,Shank-interacting protein-like 1,hSIPL1

Sequence modifications

A, C, E, G: 480 - 639 (UniProt: Q96EP0)

PDB	External Database	Details
Gly 474	-	expression tag
Pro 475	-	expression tag
Gly 476	-	expression tag
Ser 477	-	expression tag
Glu 478	-	expression tag
Phe 479	-	expression tag

B, D, F, H: 206 - 309 (UniProt: Q9H0F6)

PDB	External Database	Details
Gly 202	-	expression tag
Pro 203	-	expression tag
Gly 204	-	expression tag
Ser 205	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	120700.9
All*	Total formula weight	120700.9

*Water molecules are not included.