5X0W
Molecular mechanism for the binding between Sharpin and HOIP
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97915 |
Spacegroup name | P 32 |
Unit cell lengths | 101.347, 101.347, 146.982 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.895 - 3.000 |
R-factor | 0.2632 |
Rwork | 0.261 |
R-free | 0.30230 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.742 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.897 | 3.050 |
High resolution limit [Å] | 2.999 | 2.999 |
Number of reflections | 66810 | 1681 |
<I/σ(I)> | 23.9 | 4.84 |
Completeness [%] | 99.9 | 99.78 |
Redundancy | 7.37 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 289 | 0.1 M HEPES (pH 7.3), 7% (w/v) PEG8000, 8% (v/v) ethylene glycerol |