5WT5

L-homocysteine-bound NifS from Helicobacter pylori

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Cysteine desulfurase IscS	polymer	401	44353.3	4	UniProt (O25008) Pfam (PF00266)	Helicobacter pylori (strain ATCC 700392 / 26695)	NifS
2	E (A)	ISOPROPYL ALCOHOL	non-polymer		60.1	1	Chemie (IPA)
3	F, G, H, I (A, B, C, D)	2-AMINO-4-MERCAPTO-BUTYRIC ACID	non-polymer		135.2	4	Chemie (HCS)
4	J, K, L, M (A, B, C, D)	water	water		18.0	769	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 387 (UniProt: O25008)

PDB	External Database	Details
Val 2	Leu 2	engineered mutation
Arg 138	Lys 138	engineered mutation
Val 388	-	expression tag
Asp 389	-	expression tag
Leu 390	-	expression tag
Val 391	-	expression tag
Pro 392	-	expression tag
Arg 393	-	expression tag
Gly 394	-	expression tag
Ser 395	-	expression tag
His 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	177413.4
Non-Polymers*	Number of molecules	5
	Total formula weight	600.8
All*	Total formula weight	178014.2

*Water molecules are not included.