5WT5

L-homocysteine-bound NifS from Helicobacter pylori

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006534	biological_process	cysteine metabolic process
A	0016226	biological_process	iron-sulfur cluster assembly
A	0016740	molecular_function	transferase activity
A	0030170	molecular_function	pyridoxal phosphate binding
A	0031071	molecular_function	cysteine desulfurase activity
A	0044571	biological_process	[2Fe-2S] cluster assembly
A	0046872	molecular_function	metal ion binding
A	0051536	molecular_function	iron-sulfur cluster binding
A	0051537	molecular_function	2 iron, 2 sulfur cluster binding
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006534	biological_process	cysteine metabolic process
B	0016226	biological_process	iron-sulfur cluster assembly
B	0016740	molecular_function	transferase activity
B	0030170	molecular_function	pyridoxal phosphate binding
B	0031071	molecular_function	cysteine desulfurase activity
B	0044571	biological_process	[2Fe-2S] cluster assembly
B	0046872	molecular_function	metal ion binding
B	0051536	molecular_function	iron-sulfur cluster binding
B	0051537	molecular_function	2 iron, 2 sulfur cluster binding
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006534	biological_process	cysteine metabolic process
C	0016226	biological_process	iron-sulfur cluster assembly
C	0016740	molecular_function	transferase activity
C	0030170	molecular_function	pyridoxal phosphate binding
C	0031071	molecular_function	cysteine desulfurase activity
C	0044571	biological_process	[2Fe-2S] cluster assembly
C	0046872	molecular_function	metal ion binding
C	0051536	molecular_function	iron-sulfur cluster binding
C	0051537	molecular_function	2 iron, 2 sulfur cluster binding
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006534	biological_process	cysteine metabolic process
D	0016226	biological_process	iron-sulfur cluster assembly
D	0016740	molecular_function	transferase activity
D	0030170	molecular_function	pyridoxal phosphate binding
D	0031071	molecular_function	cysteine desulfurase activity
D	0044571	biological_process	[2Fe-2S] cluster assembly
D	0046872	molecular_function	metal ion binding
D	0051536	molecular_function	iron-sulfur cluster binding
D	0051537	molecular_function	2 iron, 2 sulfur cluster binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	binding site for residue IPA A 501

Chain	Residue
A	TYR56
A	ILE68

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site_id	AC2
Number of Residues	7
Details	binding site for residue HCS A 502

Chain	Residue
A	ALA11
A	HIS104
A	ASN155
A	LLP206
A	ARG354
A	HOH615
B	HOH652

---

site_id	AC3
Number of Residues	6
Details	binding site for residue HCS B 501

Chain	Residue
B	ALA11
B	HIS104
B	ASN155
B	LLP206
B	ARG354
B	HOH691

---

site_id	AC4
Number of Residues	7
Details	binding site for residue HCS C 501

Chain	Residue
C	ASN10
C	ALA11
C	HIS104
C	ASN155
C	ARG354
C	HOH759
C	HOH763

---

site_id	AC5
Number of Residues	8
Details	binding site for residue HCS D 501

Chain	Residue
D	ASN10
D	ALA11
D	HIS104
D	ASN155
D	ARG354
D	HOH641
D	HOH765
D	HOH776

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Cysteine persulfide intermediate => ECO:0000255|HAMAP-Rule:MF_00331

Chain	Residue	Details
A	CYS328
B	CYS328
C	CYS328
D	CYS328

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site_id	SWS_FT_FI2
Number of Residues	20
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00331

Chain	Residue	Details
A	ALA73
B	THR241
C	ALA73
C	ASN155
C	GLN183
C	SER203
C	THR241
D	ALA73
D	ASN155
D	GLN183
D	SER203
A	ASN155
D	THR241
A	GLN183
A	SER203
A	THR241
B	ALA73
B	ASN155
B	GLN183
B	SER203

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: via persulfide group => ECO:0000255|HAMAP-Rule:MF_00331

Chain	Residue	Details
A	CYS328
B	CYS328
C	CYS328
D	CYS328

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site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000255|HAMAP-Rule:MF_00331

Chain	Residue	Details
A	LLP206
B	LLP206
C	LLP206
D	LLP206

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