5WS2

Crystal structure of mpy-RNase J (mutant S247A), an archaeal RNase J from Methanolobus psychrophilus R15, complex with RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ribonuclease J	polymer	470	52164.5	2	UniProt (K4MAF9)	Methanolobus psychrophilus R15	RNase J
2	C, D (C, D)	RNA (5'-R(P*AP*AP*AP*AP*A)-3')	polymer	5	1601.1	2		unidentified
3	E, F, J, K (A, B)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
4	G, H, I, L, M... (A, B, C)	SULFATE ION	non-polymer		96.1	9	Chemie (SO4)
5	R, S, T, U (A, B, C, D)	water	water		18.0	312	Chemie (HOH)

Sequence modifications

A, B: 2 - 448 (UniProt: K4MAF9)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Leu -8	-	expression tag
Val -7	-	expression tag
Pro -6	-	expression tag
Arg -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
His -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag
Ala 247	Ser 247	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	107531.1
Non-Polymers*	Number of molecules	13
	Total formula weight	1126.2
All*	Total formula weight	108657.3

*Water molecules are not included.