5WS2

Crystal structure of mpy-RNase J (mutant S247A), an archaeal RNase J from Methanolobus psychrophilus R15, complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-23
Detector	PSI PILATUS 6M
Wavelength(s)	0.97776
Spacegroup name	P 65 2 2
Unit cell lengths	168.610, 168.610, 165.868
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.674 - 2.398
R-factor	0.1745
Rwork	0.173
R-free	0.20850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hab
RMSD bond length	0.009
RMSD bond angle	1.182
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.172	0.763
Number of reflections	54889
<I/σ(I)>	17.3	5
Completeness [%]	100.0	100
Redundancy	16.5	17.2
CC(1/2)	0.997	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.01M MgSO4, 0.05M Sodium cacodylate trihydrate, 2M (NH4)2SO4