5WS2
Crystal structure of mpy-RNase J (mutant S247A), an archaeal RNase J from Methanolobus psychrophilus R15, complex with RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-23 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97776 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 168.610, 168.610, 165.868 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.674 - 2.398 |
R-factor | 0.1745 |
Rwork | 0.173 |
R-free | 0.20850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hab |
RMSD bond length | 0.009 |
RMSD bond angle | 1.182 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.172 | 0.763 |
Number of reflections | 54889 | |
<I/σ(I)> | 17.3 | 5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 16.5 | 17.2 |
CC(1/2) | 0.997 | 0.903 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.01M MgSO4, 0.05M Sodium cacodylate trihydrate, 2M (NH4)2SO4 |