5WLW

Crystal Structure of the Human Mitochondrial Cysteine Desulfurase with active Cysteine Loop within ISCU1 active site, coordinating Zn ion. Complexed with human ISD11 and E. coli ACP1 at 3.3A.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Cysteine desulfurase, mitochondrial	polymer	406	45081.6	2	UniProt (Q9Y697) Pfam (PF00266)	Homo sapiens (Human)
2	B, F (B, F)	LYR motif-containing protein 4	polymer	91	10763.5	2	UniProt (Q9HD34) Pfam (PF05347)	Homo sapiens (Human)
3	C, G (C, G)	Acyl carrier protein	polymer	77	8514.3	2	UniProt (B7MJ81) Pfam (PF00550) UniProt (by SIFTS) (P0A6A8)	Escherichia coli O45:K1 (strain S88 / ExPEC)	ACP
4	D, H (D, H)	Iron-sulfur cluster assembly enzyme ISCU, mitochondrial	polymer	150	16337.8	2	UniProt (Q9H1K1) Pfam (PF01592)	Homo sapiens (Human)	NifU-like N-terminal domain-containing protein,NifU-like protein
5	I, L (A, E)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
6	J, M (C, G)	S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate	non-polymer		540.7	2	Chemie (8Q1)
7	K, N (D, H)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, E: 56 - 457 (UniProt: Q9Y697)

PDB	External Database	Details
Met 52	-	initiating methionine
Gly 53	-	expression tag
Ser 54	-	expression tag
Ser 55	-	expression tag

B, F: 1 - 91 (UniProt: Q9HD34)

PDB	External Database	Details
Ala 11	Ser 11	variant

D, H: 1 - 142 (UniProt: Q9H1K1)

PDB	External Database	Details
Ile 115	Met 115	engineered mutation
Glu 143	-	expression tag
Leu 144	-	expression tag
His 145	-	expression tag
His 146	-	expression tag
His 147	-	expression tag
His 148	-	expression tag
His 149	-	expression tag
His 150	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	161394.3
Non-Polymers*	Number of molecules	6
	Total formula weight	1706.4
All*	Total formula weight	163100.7

*Water molecules are not included.