5WLW
Crystal Structure of the Human Mitochondrial Cysteine Desulfurase with active Cysteine Loop within ISCU1 active site, coordinating Zn ion. Complexed with human ISD11 and E. coli ACP1 at 3.3A.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, E | Cysteine desulfurase, mitochondrial | polymer | 406 | 45081.6 | 2 | UniProt (Q9Y697) Pfam (PF00266) In PDB | Homo sapiens (Human) | |
2 | B, F | LYR motif-containing protein 4 | polymer | 91 | 10763.5 | 2 | UniProt (Q9HD34) Pfam (PF05347) In PDB | Homo sapiens (Human) | |
3 | C, G | Acyl carrier protein | polymer | 77 | 8514.3 | 2 | UniProt (B7MJ81) Pfam (PF00550) UniProt (by SIFTS) (P0A6A8) In PDB | Escherichia coli O45:K1 (strain S88 / ExPEC) | ACP |
4 | D, H | Iron-sulfur cluster assembly enzyme ISCU, mitochondrial | polymer | 150 | 16337.8 | 2 | UniProt (Q9H1K1) Pfam (PF01592) In PDB | Homo sapiens (Human) | NifU-like N-terminal domain-containing protein,NifU-like protein |
5 | A, E | PYRIDOXAL-5'-PHOSPHATE | non-polymer | 247.1 | 2 | Chemie (PLP) | |||
6 | C, G | S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate | non-polymer | 540.7 | 2 | Chemie (8Q1) | |||
7 | D, H | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
A, E: 56 - 457 (UniProt: Q9Y697)
B, F: 1 - 91 (UniProt: Q9HD34)
D, H: 1 - 142 (UniProt: Q9H1K1)
PDB | External Database | Details |
---|---|---|
Met 52 | - | initiating methionine |
Gly 53 | - | expression tag |
Ser 54 | - | expression tag |
Ser 55 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ala 11 | Ser 11 | variant |
PDB | External Database | Details |
---|---|---|
Ile 115 | Met 115 | engineered mutation |
Glu 143 | - | expression tag |
Leu 144 | - | expression tag |
His 145 | - | expression tag |
His 146 | - | expression tag |
His 147 | - | expression tag |
His 148 | - | expression tag |
His 149 | - | expression tag |
His 150 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 161394.3 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 1706.4 | |
All* | Total formula weight | 163100.7 |