5WLW

Crystal Structure of the Human Mitochondrial Cysteine Desulfurase with active Cysteine Loop within ISCU1 active site, coordinating Zn ion. Complexed with human ISD11 and E. coli ACP1 at 3.3A.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0030170	molecular_function	pyridoxal phosphate binding
A	0031071	molecular_function	cysteine desulfurase activity
A	0044571	biological_process	[2Fe-2S] cluster assembly
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0016226	biological_process	iron-sulfur cluster assembly
B	0016604	cellular_component	nuclear body
B	0042803	molecular_function	protein homodimerization activity
B	0044571	biological_process	[2Fe-2S] cluster assembly
B	0044572	biological_process	[4Fe-4S] cluster assembly
B	0099128	cellular_component	mitochondrial [2Fe-2S] assembly complex
B	1990221	cellular_component	L-cysteine desulfurase complex
B	1990229	cellular_component	iron-sulfur cluster assembly complex
C	0000035	molecular_function	acyl binding
C	0000036	molecular_function	acyl carrier activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006633	biological_process	fatty acid biosynthetic process
C	0008289	molecular_function	lipid binding
C	0008610	biological_process	lipid biosynthetic process
C	0009245	biological_process	lipid A biosynthetic process
C	0009410	biological_process	response to xenobiotic stimulus
C	0031177	molecular_function	phosphopantetheine binding
D	0005506	molecular_function	iron ion binding
D	0016226	biological_process	iron-sulfur cluster assembly
D	0051536	molecular_function	iron-sulfur cluster binding
E	0030170	molecular_function	pyridoxal phosphate binding
E	0031071	molecular_function	cysteine desulfurase activity
E	0044571	biological_process	[2Fe-2S] cluster assembly
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005739	cellular_component	mitochondrion
F	0005759	cellular_component	mitochondrial matrix
F	0016226	biological_process	iron-sulfur cluster assembly
F	0016604	cellular_component	nuclear body
F	0042803	molecular_function	protein homodimerization activity
F	0044571	biological_process	[2Fe-2S] cluster assembly
F	0044572	biological_process	[4Fe-4S] cluster assembly
F	0099128	cellular_component	mitochondrial [2Fe-2S] assembly complex
F	1990221	cellular_component	L-cysteine desulfurase complex
F	1990229	cellular_component	iron-sulfur cluster assembly complex
G	0000035	molecular_function	acyl binding
G	0000036	molecular_function	acyl carrier activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006633	biological_process	fatty acid biosynthetic process
G	0008289	molecular_function	lipid binding
G	0008610	biological_process	lipid biosynthetic process
G	0009245	biological_process	lipid A biosynthetic process
G	0009410	biological_process	response to xenobiotic stimulus
G	0031177	molecular_function	phosphopantetheine binding
H	0005506	molecular_function	iron ion binding
H	0016226	biological_process	iron-sulfur cluster assembly
H	0051536	molecular_function	iron-sulfur cluster binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	binding site for residue PLP A 501

Chain	Residue
A	GLY126
E	THR295
A	ALA127
A	THR128
A	HIS156
A	MET203
A	ASP232
A	ALA234
A	GLN235
A	HIS257

---

site_id	AC2
Number of Residues	9
Details	binding site for residue 8Q1 C 301

Chain	Residue
B	ARG6
B	LEU10
B	ILE36
B	ALA39
B	PHE40
B	ASN43
B	VAL46
B	LEU55
B	ILE66

---

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN D 201

Chain	Residue
A	CYS381
D	ASP46
D	CYS70
D	HIS112

---

site_id	AC4
Number of Residues	10
Details	binding site for residue PLP E 501

Chain	Residue
A	THR295
E	GLY126
E	ALA127
E	THR128
E	HIS156
E	ASN207
E	ASP232
E	ALA234
E	GLN235
E	HIS257

---

site_id	AC5
Number of Residues	10
Details	binding site for residue 8Q1 G 301

Chain	Residue
F	ARG6
F	LEU10
F	MET16
F	PHE40
F	ASN43
F	VAL46
F	LEU55
F	ALA59
F	ASP62
F	ILE66

---

site_id	AC6
Number of Residues	4
Details	binding site for residue ZN H 201

Chain	Residue
E	CYS381
H	ASP46
H	CYS70
H	HIS112

---

Functional Information from PROSITE/UniProt

site_id	PS00012
Number of Residues	16
Details	PHOSPHOPANTETHEINE Phosphopantetheine attachment site. DLGADSLDTVELVMAL

Chain	Residue	Details
C	ASP31-LEU46

---

site_id	PS00595
Number of Residues	20
Details	AA_TRANSFER_CLASS_5 Aminotransferases class-V pyridoxal-phosphate attachment site. IDLMsiSGHKiygpk.GvGaI

Chain	Residue	Details
A	ILE249-ILE268

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Cysteine persulfide intermediate => ECO:0000250|UniProtKB:Q9D7P6

Chain	Residue	Details
D	CYS44
H	CYS44
F	ARG6
F	LYS44

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Cysteine persulfide intermediate => ECO:0000250|UniProtKB:O29689

Chain	Residue	Details
D	CYS113
H	CYS113

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:31101807, ECO:0007744|PDB:6NZU

Chain	Residue	Details
D	ASP46
D	CYS70
D	CYS113
H	ASP46
H	CYS70
H	CYS113
E	SER255
E	HIS257

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	SITE: Mediates ISCU dimerization and de novo [2Fe-2S] cluster assembly => ECO:0000269|PubMed:34824239

Chain	Residue	Details
D	GLY35
H	GLY35

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphoserine; by MTOR => ECO:0000269|PubMed:23508953

Chain	Residue	Details
D	VAL14
H	VAL14

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Cysteine persulfide => ECO:0000250|UniProtKB:Q9D7P6

Chain	Residue	Details
D	CYS44
H	CYS44

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Cysteine persulfide => ECO:0000305|PubMed:24971490

Chain	Residue	Details
D	CYS113
H	CYS113

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