5WKP

Crystal Structure of the Human mitochondrial Cysteine Desulfurase in complex with ISD11 and Iron-Sulfur Cluster Scaffold Protein ISCU1, and E. coli ACP1 protein at 3.15A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Cysteine desulfurase, mitochondrial	polymer	406	45081.6	2	UniProt (Q9Y697) Pfam (PF00266) In PDB	Homo sapiens (Human)
2	B, F	LYR motif-containing protein 4	polymer	91	10763.5	2	UniProt (Q9HD34) Pfam (PF05347) In PDB	Homo sapiens (Human)
3	C, G	Acyl carrier protein	polymer	77	8514.3	2	UniProt (B7MJ81) Pfam (PF00550) UniProt (by SIFTS) (P0A6A8) In PDB	Escherichia coli	ACP
4	D, H	Iron-sulfur cluster assembly enzyme ISCU, mitochondrial	polymer	150	16337.8	2	UniProt (Q9H1K1) Pfam (PF01592) In PDB	Homo sapiens (Human)	NifU-like N-terminal domain-containing protein,NifU-like protein
5	A, E	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
6	C, G	S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate	non-polymer		540.7	2	Chemie (8Q1)

Sequence modifications

A, E: 56 - 457 (UniProt: Q9Y697)

PDB	External Database	Details
Met 52	-	initiating methionine
Gly 53	-	expression tag
Ser 54	-	expression tag
Ser 55	-	expression tag

B, F: 1 - 91 (UniProt: Q9HD34)

PDB	External Database	Details
Ala 11	Ser 11	variant

D, H: 1 - 142 (UniProt: Q9H1K1)

PDB	External Database	Details
Ile 115	Met 115	engineered mutation
Leu 143	-	expression tag
Glu 144	-	expression tag
His 145	-	expression tag
His 146	-	expression tag
His 147	-	expression tag
His 148	-	expression tag
His 149	-	expression tag
His 150	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	161394.3
Non-Polymers*	Number of molecules	4
	Total formula weight	1575.6
All*	Total formula weight	162969.9

*Water molecules are not included.