5WKP
Crystal Structure of the Human mitochondrial Cysteine Desulfurase in complex with ISD11 and Iron-Sulfur Cluster Scaffold Protein ISCU1, and E. coli ACP1 protein at 3.15A
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, E | Cysteine desulfurase, mitochondrial | polymer | 406 | 45081.6 | 2 | UniProt (Q9Y697) Pfam (PF00266) In PDB | Homo sapiens (Human) | |
2 | B, F | LYR motif-containing protein 4 | polymer | 91 | 10763.5 | 2 | UniProt (Q9HD34) Pfam (PF05347) In PDB | Homo sapiens (Human) | |
3 | C, G | Acyl carrier protein | polymer | 77 | 8514.3 | 2 | UniProt (B7MJ81) Pfam (PF00550) UniProt (by SIFTS) (P0A6A8) In PDB | Escherichia coli | ACP |
4 | D, H | Iron-sulfur cluster assembly enzyme ISCU, mitochondrial | polymer | 150 | 16337.8 | 2 | UniProt (Q9H1K1) Pfam (PF01592) In PDB | Homo sapiens (Human) | NifU-like N-terminal domain-containing protein,NifU-like protein |
5 | A, E | PYRIDOXAL-5'-PHOSPHATE | non-polymer | 247.1 | 2 | Chemie (PLP) | |||
6 | C, G | S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate | non-polymer | 540.7 | 2 | Chemie (8Q1) |
Sequence modifications
A, E: 56 - 457 (UniProt: Q9Y697)
B, F: 1 - 91 (UniProt: Q9HD34)
D, H: 1 - 142 (UniProt: Q9H1K1)
PDB | External Database | Details |
---|---|---|
Met 52 | - | initiating methionine |
Gly 53 | - | expression tag |
Ser 54 | - | expression tag |
Ser 55 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ala 11 | Ser 11 | variant |
PDB | External Database | Details |
---|---|---|
Ile 115 | Met 115 | engineered mutation |
Leu 143 | - | expression tag |
Glu 144 | - | expression tag |
His 145 | - | expression tag |
His 146 | - | expression tag |
His 147 | - | expression tag |
His 148 | - | expression tag |
His 149 | - | expression tag |
His 150 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 161394.3 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 1575.6 | |
All* | Total formula weight | 162969.9 |