Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5WKP

Crystal Structure of the Human mitochondrial Cysteine Desulfurase in complex with ISD11 and Iron-Sulfur Cluster Scaffold Protein ISCU1, and E. coli ACP1 protein at 3.15A

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0030170	molecular_function	pyridoxal phosphate binding
A	0031071	molecular_function	cysteine desulfurase activity
A	0044571	biological_process	[2Fe-2S] cluster assembly
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0016226	biological_process	iron-sulfur cluster assembly
B	0016604	cellular_component	nuclear body
B	0042803	molecular_function	protein homodimerization activity
B	0044571	biological_process	[2Fe-2S] cluster assembly
B	0044572	biological_process	[4Fe-4S] cluster assembly
B	0099128	cellular_component	mitochondrial [2Fe-2S] assembly complex
B	1990221	cellular_component	L-cysteine desulfurase complex
B	1990229	cellular_component	iron-sulfur cluster assembly complex
C	0000035	molecular_function	acyl binding
C	0000036	molecular_function	acyl carrier activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006633	biological_process	fatty acid biosynthetic process
C	0008289	molecular_function	lipid binding
C	0008610	biological_process	lipid biosynthetic process
C	0009245	biological_process	lipid A biosynthetic process
C	0009410	biological_process	response to xenobiotic stimulus
C	0031177	molecular_function	phosphopantetheine binding
D	0005506	molecular_function	iron ion binding
D	0016226	biological_process	iron-sulfur cluster assembly
D	0051536	molecular_function	iron-sulfur cluster binding
E	0030170	molecular_function	pyridoxal phosphate binding
E	0031071	molecular_function	cysteine desulfurase activity
E	0044571	biological_process	[2Fe-2S] cluster assembly
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005739	cellular_component	mitochondrion
F	0005759	cellular_component	mitochondrial matrix
F	0016226	biological_process	iron-sulfur cluster assembly
F	0016604	cellular_component	nuclear body
F	0042803	molecular_function	protein homodimerization activity
F	0044571	biological_process	[2Fe-2S] cluster assembly
F	0044572	biological_process	[4Fe-4S] cluster assembly
F	0099128	cellular_component	mitochondrial [2Fe-2S] assembly complex
F	1990221	cellular_component	L-cysteine desulfurase complex
F	1990229	cellular_component	iron-sulfur cluster assembly complex
G	0000035	molecular_function	acyl binding
G	0000036	molecular_function	acyl carrier activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006633	biological_process	fatty acid biosynthetic process
G	0008289	molecular_function	lipid binding
G	0008610	biological_process	lipid biosynthetic process
G	0009245	biological_process	lipid A biosynthetic process
G	0009410	biological_process	response to xenobiotic stimulus
G	0031177	molecular_function	phosphopantetheine binding
H	0005506	molecular_function	iron ion binding
H	0016226	biological_process	iron-sulfur cluster assembly
H	0051536	molecular_function	iron-sulfur cluster binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	binding site for residue PLP A 501

Chain	Residue
A	GLY126
A	ALA127
A	THR128
A	HIS156
A	ASP232
A	ALA234
A	GLN235
A	HIS257
E	THR295

site_id	AC2
Number of Residues	6
Details	binding site for residue 8Q1 C 301

Chain	Residue
B	VAL9
B	ALA39
B	PHE40
B	ASN43
B	VAL46
B	ILE52

site_id	AC3
Number of Residues	9
Details	binding site for residue PLP E 501

Chain	Residue
A	THR295
E	GLY126
E	ALA127
E	THR128
E	HIS156
E	ASP232
E	ALA234
E	GLN235
E	HIS257

site_id	AC4
Number of Residues	9
Details	binding site for residue 8Q1 G 301

Chain	Residue
F	ARG6
F	VAL9
F	LEU10
F	MET16
F	ALA39
F	ASN43
F	VAL46
F	ILE52
F	LEU55

Functional Information from PROSITE/UniProt

site_id	PS00012
Number of Residues	16
Details	PHOSPHOPANTETHEINE Phosphopantetheine attachment site. DLGADSLDTVELVMAL

Chain	Residue	Details
C	ASP31-LEU46

site_id	PS00595
Number of Residues	20
Details	AA_TRANSFER_CLASS_5 Aminotransferases class-V pyridoxal-phosphate attachment site. IDLMsiSGHKiygpk.GvGaI

Chain	Residue	Details
A	ILE249-ILE268

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Cysteine persulfide intermediate => ECO:0000250\|UniProtKB:Q9D7P6

Chain	Residue	Details
D	CYS44
H	CYS44
F	ARG6
F	LYS44

site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Cysteine persulfide intermediate => ECO:0000250\|UniProtKB:O29689

Chain	Residue	Details
D	CYS113
H	CYS113

site_id	SWS_FT_FI3
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269\|PubMed:31101807, ECO:0007744\|PDB:6NZU

Chain	Residue	Details
D	ASP46
D	CYS70
D	CYS113
H	ASP46
H	CYS70
H	CYS113
E	SER255
E	HIS257

site_id	SWS_FT_FI4
Number of Residues	2
Details	SITE: Mediates ISCU dimerization and de novo [2Fe-2S] cluster assembly => ECO:0000269\|PubMed:34824239

Chain	Residue	Details
D	GLY35
H	GLY35

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphoserine; by MTOR => ECO:0000269\|PubMed:23508953

Chain	Residue	Details
D	VAL14
H	VAL14

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Cysteine persulfide => ECO:0000250\|UniProtKB:Q9D7P6

Chain	Residue	Details
D	CYS44
H	CYS44

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Cysteine persulfide => ECO:0000305\|PubMed:24971490

Chain	Residue	Details
D	CYS113
H	CYS113

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)