5WKP

Crystal Structure of the Human mitochondrial Cysteine Desulfurase in complex with ISD11 and Iron-Sulfur Cluster Scaffold Protein ISCU1, and E. coli ACP1 protein at 3.15A

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0030170	molecular_function	pyridoxal phosphate binding
A	0031071	molecular_function	cysteine desulfurase activity
A	0044571	biological_process	[2Fe-2S] cluster assembly
B	0005198	molecular_function	structural molecule activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0016226	biological_process	iron-sulfur cluster assembly
B	0016604	cellular_component	nuclear body
B	0042803	molecular_function	protein homodimerization activity
B	0044571	biological_process	[2Fe-2S] cluster assembly
B	0044572	biological_process	[4Fe-4S] cluster assembly
B	0060090	molecular_function	molecular adaptor activity
B	0099128	cellular_component	mitochondrial [2Fe-2S] assembly complex
B	1990221	cellular_component	L-cysteine desulfurase complex
C	0000035	molecular_function	acyl binding
C	0000036	molecular_function	acyl carrier activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006629	biological_process	lipid metabolic process
C	0006631	biological_process	fatty acid metabolic process
C	0006633	biological_process	fatty acid biosynthetic process
C	0008289	molecular_function	lipid binding
C	0008610	biological_process	lipid biosynthetic process
C	0009245	biological_process	lipid A biosynthetic process
C	0009410	biological_process	response to xenobiotic stimulus
C	0016020	cellular_component	membrane
C	0031177	molecular_function	phosphopantetheine binding
D	0005506	molecular_function	iron ion binding
D	0016226	biological_process	iron-sulfur cluster assembly
D	0051536	molecular_function	iron-sulfur cluster binding
E	0030170	molecular_function	pyridoxal phosphate binding
E	0031071	molecular_function	cysteine desulfurase activity
E	0044571	biological_process	[2Fe-2S] cluster assembly
F	0005198	molecular_function	structural molecule activity
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005739	cellular_component	mitochondrion
F	0005759	cellular_component	mitochondrial matrix
F	0016226	biological_process	iron-sulfur cluster assembly
F	0016604	cellular_component	nuclear body
F	0042803	molecular_function	protein homodimerization activity
F	0044571	biological_process	[2Fe-2S] cluster assembly
F	0044572	biological_process	[4Fe-4S] cluster assembly
F	0060090	molecular_function	molecular adaptor activity
F	0099128	cellular_component	mitochondrial [2Fe-2S] assembly complex
F	1990221	cellular_component	L-cysteine desulfurase complex
G	0000035	molecular_function	acyl binding
G	0000036	molecular_function	acyl carrier activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006629	biological_process	lipid metabolic process
G	0006631	biological_process	fatty acid metabolic process
G	0006633	biological_process	fatty acid biosynthetic process
G	0008289	molecular_function	lipid binding
G	0008610	biological_process	lipid biosynthetic process
G	0009245	biological_process	lipid A biosynthetic process
G	0009410	biological_process	response to xenobiotic stimulus
G	0016020	cellular_component	membrane
G	0031177	molecular_function	phosphopantetheine binding
H	0005506	molecular_function	iron ion binding
H	0016226	biological_process	iron-sulfur cluster assembly
H	0051536	molecular_function	iron-sulfur cluster binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	binding site for residue PLP A 501

Chain	Residue
A	GLY126
A	ALA127
A	THR128
A	HIS156
A	ASP232
A	ALA234
A	GLN235
A	HIS257
E	THR295

---

site_id	AC2
Number of Residues	6
Details	binding site for residue 8Q1 C 301

Chain	Residue
B	VAL9
B	ALA39
B	PHE40
B	ASN43
B	VAL46
B	ILE52

---

site_id	AC3
Number of Residues	9
Details	binding site for residue PLP E 501

Chain	Residue
A	THR295
E	GLY126
E	ALA127
E	THR128
E	HIS156
E	ASP232
E	ALA234
E	GLN235
E	HIS257

---

site_id	AC4
Number of Residues	9
Details	binding site for residue 8Q1 G 301

Chain	Residue
F	ARG6
F	VAL9
F	LEU10
F	MET16
F	ALA39
F	ASN43
F	VAL46
F	ILE52
F	LEU55

---

Functional Information from PROSITE/UniProt

site_id	PS00012
Number of Residues	16
Details	PHOSPHOPANTETHEINE Phosphopantetheine attachment site. DLGADSLDTVELVMAL

Chain	Residue	Details
C	ASP31-LEU46

---

site_id	PS00595
Number of Residues	20
Details	AA_TRANSFER_CLASS_5 Aminotransferases class-V pyridoxal-phosphate attachment site. IDLMsiSGHKiygpk.GvGaI

Chain	Residue	Details
A	ILE249-ILE268

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"29097656","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34824239","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5WKP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5WLW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6UXE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6W1D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6WI2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6WIH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7RTK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"29097656","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"31101807","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34824239","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5WKP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5WLW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NZU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6UXE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6W1D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6WI2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6WIH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7RTK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"29097656","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"31101807","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5WKP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5WLW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NZU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"N6-(pyridoxal phosphate)lysine","evidences":[{"source":"PubMed","id":"29097656","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"31101807","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34824239","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5WKP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5WLW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NZU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6UXE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6W1D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6WI2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6WIH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7RTK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"31664822","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6ODD","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"Q8K215","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Modified residue: {"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"source":"PROSITE-ProRule","id":"PRU00258","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"4882207","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Active site: {"description":"Cysteine persulfide intermediate","evidences":[{"source":"UniProtKB","id":"Q9D7P6","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Active site: {"description":"Cysteine persulfide intermediate","evidences":[{"source":"UniProtKB","id":"O29689","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"31101807","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NZU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	Modified residue: {"description":"Cysteine persulfide","evidences":[{"source":"UniProtKB","id":"Q9D7P6","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	Modified residue: {"description":"Cysteine persulfide","evidences":[{"source":"PubMed","id":"24971490","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"29097656","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5WLW","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---