5WBU

Crystal structure of mTOR(deltaN)-mLST8-PRAS40(alpha-helix & beta-strand) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (B, A)	Serine/threonine-protein kinase mTOR	polymer	1177	134865.5	2	UniProt (P42345) Pfam (PF02259) Pfam (PF23593) Pfam (PF08771) Pfam (PF00454) Pfam (PF02260)	Homo sapiens (Human)	FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP12-rapamycin complex-associated protein,Mammalian target of rapamycin,mTOR,Mechanistic target of rapamycin,Rapamycin and FKBP12 target 1,Rapamycin target protein 1
2	B, F (D, C)	Target of rapamycin complex subunit LST8	polymer	328	36054.2	2	UniProt (Q9BVC4) Pfam (PF00400)	Homo sapiens (Human)	TORC subunit LST8,G protein beta subunit-like,Protein GbetaL,Mammalian lethal with SEC13 protein 8,mLST8
3	C, D, G, H (P, R, O, Q)	Proline-rich AKT1 substrate 1	polymer	88	9848.0	4	UniProt (Q96B36) Pfam (PF15798)	Homo sapiens (Human)	40 kDa proline-rich AKT substrate

Sequence modifications

B, A: 1376 - 2549 (UniProt: P42345)

PDB	External Database	Details
Gly 1373	-	expression tag
Thr 1374	-	expression tag
Gly 1375	-	expression tag

D, C: 1 - 326 (UniProt: Q9BVC4)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

P, R, O, Q: 173 - 256 (UniProt: Q96B36)

PDB	External Database	Details
Gly 169	-	expression tag
Ser 170	-	expression tag
Gly 171	-	expression tag
Arg 172	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	381231.5
All*	Total formula weight	381231.5

*Water molecules are not included.