5WBU
Crystal structure of mTOR(deltaN)-mLST8-PRAS40(alpha-helix & beta-strand) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 139.400, 163.200, 207.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 3.420 |
| R-factor | 0.23531 |
| Rwork | 0.235 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jsn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.302 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 80.000 | 80.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 7.320 | 3.400 |
| Rmerge | 0.136 | 0.040 | 0.590 |
| Rmeas | 0.158 | 0.045 | 0.711 |
| Rpim | 0.079 | 0.021 | 0.386 |
| Total number of observations | 227760 | ||
| Number of reflections | 61593 | 5497 | |
| <I/σ(I)> | 4.5 | ||
| Completeness [%] | 95.5 | 97.9 | 86.5 |
| Redundancy | 3.7 | 4.2 | 2.8 |
| CC(1/2) | 0.999 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 8000 |
