5VZT

Crystal structure of the Skp1-FBXO31 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	S-phase kinase-associated protein 1	polymer	149	16827.1	2	UniProt (P63208) Pfam (PF03931) Pfam (PF01466) In PDB	Homo sapiens (Human)	Cyclin-A/CDK2-associated protein p19,p19A,Organ of Corti protein 2,OCP-2,Organ of Corti protein II,OCP-II,RNA polymerase II elongation factor-like protein,SIII,Transcription elongation factor B polypeptide 1-like,p19skp1
2	B, D	F-box only protein 31	polymer	488	55730.1	2	UniProt (Q5XUX0) Pfam (PF12937) Pfam (PF12014) In PDB	Homo sapiens (Human)
3	B, D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	B, D	2,3-DIHYDROXY-1,4-DITHIOBUTANE	non-polymer		154.3	2	Chemie (DTT)
5	B, D	PHOSPHATE ION	non-polymer		95.0	8	Chemie (PO4)
6		water	water		18.0	82	Chemie (HOH)

Sequence modifications

A, C: 1001 - 1163 (UniProt: P63208)

PDB	External Database	Details
Ala 1002	Pro 2	engineered mutation
-	Asp 38	deletion
-	Glu 39	deletion
-	Gly 40	deletion
-	Asp 41	deletion
-	Asp 42	deletion
-	Asp 43	deletion
-	Pro 70	deletion
-	Pro 71	deletion
-	Pro 72	deletion
-	Glu 73	deletion
-	Asp 74	deletion
-	Asp 75	deletion
-	Glu 76	deletion
-	Asn 77	deletion
Gly 1078	Lys 78	see remark 999
Gly 1079	Glu 79	see remark 999
Ser 1080	Lys 80	see remark 999
Gly 1081	Arg 81	see remark 999

B, D: 66 - 539 (UniProt: Q5XUX0)

PDB	External Database	Details
Met 52	-	expression tag
Ala 53	-	expression tag
Ser 54	-	expression tag
Trp 55	-	expression tag
Ser 56	-	expression tag
His 57	-	expression tag
Pro 58	-	expression tag
Gln 59	-	expression tag
Phe 60	-	expression tag
Glu 61	-	expression tag
Lys 62	-	expression tag
Ser 63	-	expression tag
Gly 64	-	expression tag
Arg 65	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	145114.5
Non-Polymers*	Number of molecules	12
	Total formula weight	1199.1
All*	Total formula weight	146313.6

*Water molecules are not included.