5VZT
Crystal structure of the Skp1-FBXO31 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.521, 156.310, 154.596 |
| Unit cell angles | 90.00, 103.62, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.1976 |
| Rwork | 0.195 |
| R-free | 0.24230 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.956 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
| Rmerge | 0.069 | 0.028 | 0.647 |
| Total number of observations | 413619 | ||
| Number of reflections | 54754 | ||
| <I/σ(I)> | 11.2 | ||
| Completeness [%] | 99.2 | 99.6 | 91.5 |
| Redundancy | 7.6 | 7.6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 277 | 100 mM sodium citrate (pH 5.3), 0.27 M ammonium acetate, 13-17% MPD, 0.02 M CaCl2 |
