5VZT

Crystal structure of the Skp1-FBXO31 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	C 1 2 1
Unit cell lengths	87.521, 156.310, 154.596
Unit cell angles	90.00, 103.62, 90.00

Refinement procedure

Resolution	50.000 - 2.700
R-factor	0.1976
Rwork	0.195
R-free	0.24230
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.956
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.069	0.028	0.647
Total number of observations	413619
Number of reflections	54754
<I/σ(I)>	11.2
Completeness [%]	99.2	99.6	91.5
Redundancy	7.6	7.6	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	277	100 mM sodium citrate (pH 5.3), 0.27 M ammonium acetate, 13-17% MPD, 0.02 M CaCl2