5VW1
Crystal structure of SpyCas9-sgRNA-AcrIIA4 ternary complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1370 | 158732.9 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) In PDB | Streptococcus pyogenes serotype M1 | SpCas9,SpyCas9 |
2 | C | sgRNA | polymer | 85 | 27400.3 | 1 | Listeria monocytogenes | ||
3 | B | anti-CRISPR protein AcrIIA4 | polymer | 89 | 10326.2 | 1 | UniProt (A0A0E0UT28) In PDB | Listeria monocytogenes serotype 4a (strain M7) | |
4 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 2 | Chemie (PEG) | |||
5 | A, C | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 2 | Chemie (PG4) | |||
6 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
7 | C | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
8 | water | water | 18.0 | 190 | Chemie (HOH) |
Sequence modifications
A: 1 - 1368 (UniProt: Q99ZW2)
B: 1 - 87 (UniProt: A0A0E0UT28)
PDB | External Database | Details |
---|---|---|
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
Ala 10 | Asp 10 | engineered mutation |
Ala 840 | His 840 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
Asn 4 | Ser 4 | see REMARK 999 |
Asp 5 | Glu 5 | see REMARK 999 |
Thr 16 | Ala 16 | see REMARK 999 |
Lys 18 | Arg 18 | see REMARK 999 |
Ser 20 | Glu 20 | see REMARK 999 |
Ser 24 | Asp 24 | see REMARK 999 |
Gln 29 | Lys 29 | see REMARK 999 |
Arg 33 | Asp 33 | see REMARK 999 |
Asn 35 | Asp 35 | see REMARK 999 |
Glu 47 | Lys 47 | see REMARK 999 |
Val 52 | Ala 52 | see REMARK 999 |
Ile 56 | Ala 56 | see REMARK 999 |
Ala 58 | Thr 58 | see REMARK 999 |
Gln 65 | Lys 65 | see REMARK 999 |
Thr 79 | Ser 79 | see REMARK 999 |
Thr 81 | Ile 81 | see REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 196459.4 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 687.1 | |
All* | Total formula weight | 197146.5 |