5VVL

Cas1-Cas2 bound to full-site mimic with Ni

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	CRISPR-associated endonuclease Cas1	polymer	308	33570.8	4	UniProt (Q46896) Pfam (PF01867)	Escherichia coli (strain K12)
2	E, F (E, F)	CRISPR-associated endoribonuclease Cas2	polymer	103	11553.3	2	UniProt (P45956) Pfam (PF09707)	Escherichia coli (strain K12)
3	G (G)	DNA (11-MER)	polymer	11	3319.2	1		synthetic construct
4	H (H)	DNA (11-MER)	polymer	11	3343.2	1		synthetic construct
5	I (J)	DNA (58-MER)	polymer	58	17907.4	1		synthetic construct
6	J (K)	DNA (58-MER)	polymer	58	17967.5	1		synthetic construct
7	AA, BA, CA, DA, EA... (K, A, B, C, D...)	NICKEL (II) ION	non-polymer		58.7	22	Chemie (NI)

Sequence modifications

A, B, C, D: 1 - 305 (UniProt: Q46896)

PDB	External Database	Details
Ser -2	-	expression tag
Phe -1	-	expression tag
Thr 0	-	expression tag

E, F: 1 - 94 (UniProt: P45956)

PDB	External Database	Details
Gly 95	-	expression tag
Ser 96	-	expression tag
Ser 97	-	expression tag
Glu 98	-	expression tag
Asn 99	-	expression tag
Leu 100	-	expression tag
Tyr 101	-	expression tag
Phe 102	-	expression tag
Gln 103	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	199926.9
Non-Polymers*	Number of molecules	22
	Total formula weight	1291.2
All*	Total formula weight	201218.1

*Water molecules are not included.