5VVL
Cas1-Cas2 bound to full-site mimic with Ni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-03-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.642, 197.728, 88.792 |
Unit cell angles | 90.00, 111.35, 90.00 |
Refinement procedure
Resolution | 39.504 - 3.310 |
R-factor | 0.2262 |
Rwork | 0.224 |
R-free | 0.26290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ds5 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.493 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.550 | 39.550 | 3.070 |
High resolution limit [Å] | 2.960 | 11.480 | 2.960 |
Rmerge | 0.203 | 0.037 | 4.410 |
Rmeas | 0.220 | 0.040 | 4.792 |
Rpim | 0.083 | 0.016 | 1.842 |
Total number of observations | 329343 | ||
Number of reflections | 47810 | ||
<I/σ(I)> | 7.9 | ||
Completeness [%] | 96.3 | 95.7 | 71.8 |
Redundancy | 6.9 | 6.5 | 6.3 |
CC(1/2) | 0.996 | 0.999 | 0.215 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 281 | 100 mM MES pH 6.4, 20% (w/v) PEG MME 2000, 0.2 M NaCl, 3 mM NiCl2 |