5VSI

CH1/Ckappa Fab mutant 15.1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	H	CH1/Ckappa Fab heavy chain	polymer	221	23780.3	1	UniProt (Q6GMX6) Pfam (PF07686) Pfam (PF07654) In PDB	Homo sapiens
2	L	CH1/Ckappa Fab light chain	polymer	212	23331.9	1	UniProt (Q7Z3Y4) Pfam (PF07686) Pfam (PF07654) In PDB	Homo sapiens
3	H, L	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
4	H, L	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
5		water	water		18.0	396	Chemie (HOH)

Sequence modifications

H: 1 - 107 (PDB: 5VSI)
H: 108 - 221 (UniProt: Q6GMX6)

PDB	External Database	Details
Ala 151	Lys 165	engineered mutation
Ala 187	Ser 201	engineered mutation
Glu 217	Lys 231	engineered mutation

L: 1 - 106 (PDB: 5VSI)
L: 107 - 212 (UniProt: Q7Z3Y4)

PDB	External Database	Details
Lys 122	Glu 145	engineered mutation
Arg 130	Ser 153	engineered mutation
Ile 175	Ser 198	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	47112.3
Non-Polymers*	Number of molecules	9
	Total formula weight	728.6
All*	Total formula weight	47840.8

*Water molecules are not included.