5VK3

Apo ctPRC2 with E840A and K852D mutations in Ezh2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Polycomb Protein EED	polymer	605	66021.4	1	UniProt (G0S8H7) Pfam (PF00400) Pfam (PF22038)	Chaetomium thermophilum
2	B (B)	Histone-lysine N-methyltransferase EZH2,Polycomb protein SUZ12	polymer	937	106797.5	1	UniProt (G0SDW4) UniProt (G0RYC6) Pfam (PF18601) Pfam (PF18600) Pfam (PF21509) Pfam (PF00856) Pfam (PF09733)	Chaetomium thermophilum
3	C, D, E, F, G... (B)	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
4	K, L (A, B)	water	water		18.0	900	Chemie (HOH)

Sequence modifications

A: 1 - 565 (UniProt: G0S8H7)

PDB	External Database	Details
Met -39	-	initiating methionine
Ala -38	-	expression tag
Ser -37	-	expression tag
Ala -36	-	expression tag
Trp -35	-	expression tag
Ser -34	-	expression tag
His -33	-	expression tag
Pro -32	-	expression tag
Gln -31	-	expression tag
Phe -30	-	expression tag
Glu -29	-	expression tag
Lys -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ala -16	-	expression tag
Trp -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
Pro -12	-	expression tag
Gln -11	-	expression tag
Phe -10	-	expression tag
Glu -9	-	expression tag
Lys -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

B: 191 - 2523 (UniProt: G0SDW4)

PDB	External Database	Details
Ser 182	-	expression tag
Asn 183	-	expression tag
His 184	-	expression tag
His 185	-	expression tag
His 186	-	expression tag
His 187	-	expression tag
His 188	-	expression tag
His 189	-	expression tag
Ala 190	-	expression tag
Ala 840	Glu 840	engineered mutation
Asp 852	Lys 852	engineered mutation
Leu 2524	-	linker
Val 2525	-	linker
Pro 2526	-	linker
Arg 2527	-	linker
Gly 2528	-	linker
Ser 2529	-	linker

B: 2530 - 2691 (UniProt: G0RYC6)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	172818.8
Non-Polymers*	Number of molecules	8
	Total formula weight	523.3
All*	Total formula weight	173342.1

*Water molecules are not included.