5V6Y
Crystal structure of the human CLR:RAMP1 extracellular domain heterodimer with bound high-affinity and altered selectivity adrenomedullin variant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Maltose-binding periplasmic protein,Receptor activity-modifying protein 1,Calcitonin gene-related peptide type 1 receptor | polymer | 593 | 66304.6 | 4 | UniProt (P0AEY0) UniProt (O60894) UniProt (Q16602) Pfam (PF01547) Pfam (PF04901) Pfam (PF02793) UniProt (by SIFTS) (P0AEX9) | Escherichia coli O157:H7 | MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Calcitonin-receptor-like receptor activity-modifying protein 1,CRLR activity-modifying protein 1,CGRP type 1 receptor,Calcitonin receptor-like receptor |
| 2 | E, F, G, H (E, F, G, H) | ADM | polymer | 17 | 1901.2 | 4 | UniProt (P35318) | Homo sapiens | |
| 3 | I, J, K, L (I, J, K, L) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 4 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 4 | M, N (A, B) | water | water | 18.0 | 3 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 2 - 368 (UniProt: P0AEY0)
A, B, C, D: 1024 - 2019 (UniProt: O60894)
A, B, C, D: 2029 - 2144 (UniProt: Q16602)
E, F, G, H: 37 - 52 (UniProt: P35318)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Asn 369 | - | linker |
| Ala 370 | - | linker |
| Ala 371 | - | linker |
| Ala 372 | - | linker |
| Glu 373 | - | linker |
| Phe 374 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly 2020 | - | linker |
| Ser 2021 | - | linker |
| Ala 2022 | - | linker |
| Gly 2023 | - | linker |
| Ser 2024 | - | linker |
| Ala 2025 | - | linker |
| Gly 2026 | - | linker |
| Ser 2027 | - | linker |
| Ala 2028 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| His 2145 | - | expression tag |
| His 2146 | - | expression tag |
| His 2147 | - | expression tag |
| His 2148 | - | expression tag |
| His 2149 | - | expression tag |
| His 2150 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Trp 45 | Ser 139 | engineered mutation |
| Leu 46 | Lys 140 | engineered mutation |
| Trp 50 | Gln 144 | engineered mutation |
| Phe 52 | Tyr 146 | engineered mutation |
| Nh2 53 | - | amidation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 272822.9 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 1369.2 | |
| All* | Total formula weight | 274192.0 |
