5UTF
Crystal Structure of a Stabilized DS-SOSIP.6mut BG505 gp140 HIV-1 Env Trimer, Containing Mutations I201C-P433C (DS), L154M, Y177W, N300M, N302M, T320L, I420M in Complex with Human Antibodies PGT122 and 35O22 at 4.3 A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (G) | Envelope glycoprotein gp120 | polymer | 481 | 54191.7 | 1 | UniProt (Q2N0S6) Pfam (PF00516) | Human immunodeficiency virus 1 | |
| 2 | B (B) | Envelope glycoprotein gp41 | polymer | 153 | 17146.5 | 1 | UniProt (Q2N0S6) Pfam (PF00517) | Human immunodeficiency virus 1 | |
| 3 | C (L) | PGT122 Light chain | polymer | 213 | 22880.3 | 1 | Homo sapiens | ||
| 4 | D (H) | PGT122 Heavy chain | polymer | 235 | 25434.7 | 1 | Homo sapiens | ||
| 5 | E (D) | 35022 Heavy chain | polymer | 243 | 26170.5 | 1 | Homo sapiens | ||
| 6 | F (E) | 35022 Light Chain | polymer | 216 | 23318.8 | 1 | Homo sapiens | ||
| 7 | G (A) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1721.5 | 1 | In PDB GlyTouCan (G40702WU) | |||
| 8 | H (C) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 9 | I, K, L, M, N... (F, J, K, M, N...) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 9 | In PDB GlyTouCan (G42666HT) | |||
| 10 | J (I) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 1 | In PDB GlyTouCan (G56014GC) | |||
| 11 | O (O) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 12 | T (T) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1721.5 | 1 | In PDB GlyTouCan (G91704UR) | |||
| 13 | AA, U, V, W, X... (B, G) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 7 | Chemie (NAG) |
Sequence modifications
G: 31 - 513 (UniProt: Q2N0S6)
B: 512 - 664 (UniProt: Q2N0S6)
| PDB | External Database | Details |
|---|---|---|
| Met 154 | Leu 145 | engineered mutation |
| Trp 177 | Tyr 168 | engineered mutation |
| Cys 201 | Ile 200 | engineered mutation |
| Met 300 | Asn 299 | engineered mutation |
| Met 302 | Asn 301 | engineered mutation |
| Leu 320 | Thr 317 | engineered mutation |
| Asn 332 | Thr 330 | engineered mutation |
| Met 420 | Ile 417 | engineered mutation |
| Cys 433 | Ala 430 | engineered mutation |
| Cys 501 | Ala 498 | engineered mutation |
| Arg 509 | Glu 506 | engineered mutation |
| Arg 510 | Lys 507 | engineered mutation |
| Arg 512 | Ala 509 | engineered mutation |
| Arg 513 | Val 510 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 556 | engineered mutation |
| Cys 605 | Thr 602 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 169142.5 | |
| Branched | Number of molecules | 14 |
| Total formula weight | 9833.0 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1548.5 | |
| All* | Total formula weight | 180523.9 |
