5UTF

Crystal Structure of a Stabilized DS-SOSIP.6mut BG505 gp140 HIV-1 Env Trimer, Containing Mutations I201C-P433C (DS), L154M, Y177W, N300M, N302M, T320L, I420M in Complex with Human Antibodies PGT122 and 35O22 at 4.3 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-21
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 63
Unit cell lengths	128.380, 128.380, 313.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.982 - 3.503
R-factor	0.2198
Rwork	0.215
R-free	0.29890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tvp
RMSD bond length	0.004
RMSD bond angle	0.676
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.500
Number of reflections	18609
<I/σ(I)>	4.3
Completeness [%]	63.2
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	13.2% PEG 400, 6.6% PEG 8000, 0.1M. Sodium acetate/Acetic acid pH 4.5