5ULH

Structure of RNF165 in complex with a UbcH5b~Ub conjugate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ubiquitin-conjugating enzyme E2 D2	polymer	152	17144.5	1	UniProt (P62837) Pfam (PF00179) In PDB	Homo sapiens (Human)	(E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
2	B	Ubiquitin	polymer	76	8576.8	1	UniProt (P0CG47) Pfam (PF00240) In PDB	Homo sapiens (Human)
3	C	E3 ubiquitin-protein ligase RNF165	polymer	92	10300.7	1	UniProt (Q6ZSG1) Pfam (PF13639) In PDB	Homo sapiens (Human)	RING finger protein 165
4	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	A, B	THIOCYANATE ION	non-polymer		58.1	2	Chemie (SCN)
6	C	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7		water	water		18.0	68	Chemie (HOH)

Sequence modifications

A: 1 - 147 (UniProt: P62837)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 21	Cys 21	engineered mutation
Lys 85	Cys 85	engineered mutation
Ser 107	Cys 107	engineered mutation
Ser 111	Cys 111	engineered mutation

C: 260 - 346 (UniProt: Q6ZSG1)

PDB	External Database	Details
Gly 255	-	expression tag
Pro 256	-	expression tag
Leu 257	-	expression tag
Gly 258	-	expression tag
Ser 259	-	expression tag
-	Arg 273	deletion
-	Arg 274	deletion
-	Pro 275	deletion
-	Gln 276	deletion
-	Asp 277	deletion
Ala 313	Met 313	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	36022.1
Non-Polymers*	Number of molecules	6
	Total formula weight	431.2
All*	Total formula weight	36453.3

*Water molecules are not included.