5UHN

Crystal Structure of the Tyrosine Kinase Domain of FGF Receptor 2 harboring a N549H/E565A Double Gain-of-Function Mutation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Fibroblast growth factor receptor 2	polymer	324	37076.6	2	UniProt (P21802) Pfam (PF07714)	Homo sapiens (Human)	FGFR-2,K-sam,KGFR,Keratinocyte growth factor receptor
2	C, F (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	D, G (A, B)	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	2	Chemie (ACP)
4	E, H (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	I, J (A, B)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

A, B: 458 - 768 (UniProt: P21802)

PDB	External Database	Details
Met 445	-	initiating methionine
Gly 446	-	expression tag
Ser 447	-	expression tag
Ser 448	-	expression tag
His 449	-	expression tag
His 450	-	expression tag
His 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
Ser 455	-	expression tag
Gln 456	-	expression tag
Asp 457	-	expression tag
Ala 491	Cys 375	conflict
His 549	Asn 433	engineered mutation
Ala 565	Glu 449	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	74153.2
Non-Polymers*	Number of molecules	6
	Total formula weight	1251.2
All*	Total formula weight	75404.3

*Water molecules are not included.