5UHN

Crystal Structure of the Tyrosine Kinase Domain of FGF Receptor 2 harboring a N549H/E565A Double Gain-of-Function Mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-24
Detector	MAR CCD 165 mm
Wavelength(s)	0.97885
Spacegroup name	P 21 21 21
Unit cell lengths	67.334, 78.557, 116.546
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.279 - 2.909
R-factor	0.2597
Rwork	0.254
R-free	0.31120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2psq
RMSD bond length	0.002
RMSD bond angle	0.467
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Number of reflections	14079
<I/σ(I)>	26.5	7.2
Completeness [%]	100.0	100
Redundancy	14	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25 mM HEPES (pH 7.5), 15-25% w/v PEG 4000, 0.2-0.3 M Ammonium Sulfate