5U84

Acid ceramidase (ASAH1, aCDase) from common minke whale, Cys143Ala, uncleaved

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Acid ceramidase	polymer	384	43862.3	2	UniProt (A0A383ZFX3) Pfam (PF15508) Pfam (PF02275)	Balaenoptera acutorostrata (minke whale)	Acid CDase
2	C, E (C, E)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	2	In PDB GlyTouCan (G21290RB)
3	D (D)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
4	EA, F, FA, G, H... (B, A)	5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid	non-polymer		558.8	9	Chemie (I3C)
5	AA, BA, CA, DA, GA... (A, B)	IODIDE ION	non-polymer		126.9	34	Chemie (IOD)
6	WA, XA (A, B)	water	water		18.0	134	Chemie (HOH)

Sequence modifications

A, B: 22 - 395 (UniProt: A0A383ZFX3)

PDB	External Database	Details
Asp 12	-	expression tag
Arg 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
Lys 20	-	expression tag
Leu 21	-	expression tag
Ala 143	Cys 143	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	87724.6
Branched	Number of molecules	3
	Total formula weight	1565.5
Non-Polymers*	Number of molecules	43
	Total formula weight	9344.3
All*	Total formula weight	98634.4

*Water molecules are not included.