5U31
Crystal structure of AacC2c1-sgRNA-8mer substrate DNA ternary complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | CRISPR-associated endonuclease C2c1 | polymer | 1130 | 131747.0 | 1 | UniProt (T0D7A2) Pfam (PF22202) Pfam (PF22204) Pfam (PF22172) Pfam (PF22126) Pfam (PF22077) In PDB | Alicyclobacillus acidoterrestris | AacC2c1 |
2 | B | sgRNA | polymer | 112 | 36183.6 | 1 | Alicyclobacillus acidoterrestris | ||
3 | C | Target DNA strand | polymer | 28 | 8532.5 | 1 | Homo sapiens | ||
4 | D, E | Non-target DNA strand | polymer | 8 | 2448.6 | 2 | Homo sapiens | ||
5 | A | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) |
Sequence modifications
A: 1 - 1129 (UniProt: T0D7A2)
PDB | External Database | Details |
---|---|---|
Ser 0 | - | expression tag |
Ala 570 | Asp 570 | engineered mutation |
Ala 848 | Glu 848 | engineered mutation |
Ala 977 | Asp 977 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 181360.3 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 192.1 | |
All* | Total formula weight | 181552.4 |