5U31
Crystal structure of AacC2c1-sgRNA-8mer substrate DNA ternary complex
Functional Information from GO Data
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 A 1201 |
| Chain | Residue |
| A | TYR35 |
| A | TRP39 |
| A | ARG75 |
| A | ARG192 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 A 1202 |
| Chain | Residue |
| A | ARG643 |
| A | ARG646 |
| A | ARG766 |
| C | DG1 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 18 |
| Details | Region: {"description":"Binds sgRNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 5 |
| Details | Region: {"description":"Binds DNA protospacer adjacent motif (PAM) on target DNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | Region: {"description":"Binds non-target ssDNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 29 |
| Details | Region: {"description":"Bridge helix domain","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Active site: {"description":"For DNase activity of RuvC domain","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds DNA protospacer adjacent motif (PAM) on target DNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 9 |
| Details | Site: {"description":"Binds sgRNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds 'phosphate lock' on target strand DNA; via amide nitrogen","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds 'phosphate lock' on target strand DNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | Site: {"description":"Disrupts base stacking adjacent to scissile phosphate","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
