5U1E

Crystal structure of a methyltransferase involved in the biosynthesis of gentamicin in complex with the kanamycin B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Putative gentamicin methyltransferase	polymer	341	37395.7	1	UniProt (Q2MG72) Pfam (PF05175)	Micromonospora echinospora
2	B (A)	(1R,2S,3S,4R,6S)-4,6-DIAMINO-3-[(3-AMINO-3-DEOXY-ALPHA-D-GLUCOPYRANOSYL)OXY]-2-HYDROXYCYCLOHEXYL 2,6-DIAMINO-2,6-DIDEOXY-ALPHA-D-GLUCOPYRANOSIDE	non-polymer		483.5	1	Chemie (9CS)
3	C (A)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
4	D (A)	water	water		18.0	126	Chemie (HOH)

Sequence modifications

A: 1 - 321 (UniProt: Q2MG72)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	37395.7
Non-Polymers*	Number of molecules	2
	Total formula weight	867.9
All*	Total formula weight	38263.7

*Water molecules are not included.