5TT6

T4 RNA Ligase 1 (K99M)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	T4 RNA ligase 1	polymer	394	46040.9	1	UniProt (P00971) Pfam (PF09511) Pfam (PF20819) In PDB	Enterobacteria phage T4	Gene product 63,gp63,Rnl1
2	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3	A	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
4		water	water		18.0	93	Chemie (HOH)

Sequence modifications

A: 1 - 374 (UniProt: P00971)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ser -7	-	expression tag
Ser -6	-	expression tag
His -5	-	expression tag
Ile -4	-	expression tag
Glu -3	-	expression tag
Gly -2	-	expression tag
Arg -1	-	expression tag
His 0	-	expression tag
Met 99	Lys 99	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	46040.9
Non-Polymers*	Number of molecules	3
	Total formula weight	555.8
All*	Total formula weight	46596.7

*Water molecules are not included.