5TT6

T4 RNA Ligase 1 (K99M)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003972	molecular_function	RNA ligase (ATP) activity
A	0005524	molecular_function	ATP binding
A	0016874	molecular_function	ligase activity
A	0042245	biological_process	RNA repair
A	0046872	molecular_function	metal ion binding
A	0098004	biological_process	virus tail fiber assembly

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue MG A 401

Chain	Residue
A	ASP272
A	ATP403
A	HOH522
A	HOH531
A	HOH536

---

site_id	AC2
Number of Residues	6
Details	binding site for residue MG A 402

Chain	Residue
A	HOH535
A	HOH561
A	ATP403
A	HOH507
A	HOH508
A	HOH509

---

site_id	AC3
Number of Residues	25
Details	binding site for residue ATP A 403

Chain	Residue
A	TYR37
A	ARG54
A	MET73
A	LYS75
A	LEU97
A	THR98
A	MET99
A	GLU100
A	LEU104
A	GLU159
A	LEU179
A	VAL230
A	LYS240
A	LYS242
A	ASP272
A	MG401
A	MG402
A	HOH507
A	HOH508
A	HOH522
A	HOH525
A	HOH531
A	HOH535
A	HOH536
A	HOH561

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: N6-AMP-lysine intermediate => ECO:0000255|HAMAP-Rule:MF_04149, ECO:0000269|PubMed:3882425

Chain	Residue	Details
A	MET99

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_04149, ECO:0000269|PubMed:28223499

Chain	Residue	Details
A	TYR37
A	ARG54
A	LYS75
A	GLU159
A	LYS240
A	LYS242

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_04149, ECO:0000269|PubMed:16263720, ECO:0000269|PubMed:28223499

Chain	Residue	Details
A	ASP272

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	SITE: Essential for RNA ligase activity => ECO:0000255|HAMAP-Rule:MF_04149, ECO:0000269|PubMed:17068206

Chain	Residue	Details
A	GLU159
A	TYR246

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