5TT6
T4 RNA Ligase 1 (K99M)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.293, 39.960, 108.268 |
| Unit cell angles | 90.00, 116.36, 90.00 |
Refinement procedure
| Resolution | 48.507 - 2.187 |
| R-factor | 0.1958 |
| Rwork | 0.189 |
| R-free | 0.25940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c5u |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.053 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.055 | 0.438 |
| Number of reflections | 19235 | |
| <I/σ(I)> | 15.4 | 2 |
| Completeness [%] | 90.1 | 69.3 |
| Redundancy | 3.1 | 3.1 |
| CC(1/2) | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2 M MgCl2, 16% PEG3350 |
