5TT6
T4 RNA Ligase 1 (K99M)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97920 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 107.293, 39.960, 108.268 |
Unit cell angles | 90.00, 116.36, 90.00 |
Refinement procedure
Resolution | 48.507 - 2.187 |
R-factor | 0.1958 |
Rwork | 0.189 |
R-free | 0.25940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c5u |
RMSD bond length | 0.008 |
RMSD bond angle | 1.053 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.055 | 0.438 |
Number of reflections | 19235 | |
<I/σ(I)> | 15.4 | 2 |
Completeness [%] | 90.1 | 69.3 |
Redundancy | 3.1 | 3.1 |
CC(1/2) | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2 M MgCl2, 16% PEG3350 |