5TD5

Crystal Structure of Human APOBEC3B variant complexed with ssDNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA dC->dU-editing enzyme APOBEC-3B	polymer	190	22393.3	1	UniProt (Q9UH17) Pfam (PF05240)	Homo sapiens (Human)	A3B,Phorbolin-1-related protein,Phorbolin-2/3
2	B (C)	DNA (5'-D(P*TP*TP*CP*AP*T)-3')	polymer	7	2078.4	1		Homo sapiens
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	D, E, F, G, H... (A, C)	IODIDE ION	non-polymer		126.9	9	Chemie (IOD)
5	L, M, N, O, P... (A)	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
6	R, S (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
7	U, V (A, C)	water	water		18.0	98	Chemie (HOH)

Sequence modifications

A: 187 - 378 (UniProt: Q9UH17)

PDB	External Database	Details
Met 186	-	expression tag
Ser 200	Phe 200	engineered mutation
-	Asp 205	deletion
-	Pro 206	deletion
-	Leu 207	deletion
Gly 205	Val 208	engineered mutation
Ile 209	Leu 209	engineered mutation
Gly 210	Arg 210	engineered mutation
His 212	Arg 212	engineered mutation
Lys 213	Gln 213	engineered mutation
Ser 228	Trp 228	engineered mutation
Lys 230	Leu 230	engineered mutation
-	Ala 242	deletion
-	Lys 243	deletion
-	Asn 244	deletion
-	Leu 245	deletion
-	Leu 246	deletion
-	Cys 247	deletion
-	Gly 248	deletion
-	Phe 249	deletion
Ser 250	Tyr 250	engineered mutation
Ala 255	Glu 255	engineered mutation
Lys 308	Phe 308	engineered mutation
Leu 379	-	expression tag
Glu 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	24471.7
Non-Polymers*	Number of molecules	18
	Total formula weight	1650.9
All*	Total formula weight	26122.6

*Water molecules are not included.