5T5N
Calcium-activated chloride channel bestrophin-1 (BEST1), triple mutant: I76A, F80A, F84A; in complex with an Fab antibody fragment, chloride, and calcium
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E (A, B, C, D, E) | bestrophin-1 (BEST1) | polymer | 409 | 47420.1 | 5 | UniProt (E1C3A0) | Gallus gallus (Chicken) | |
| 2 | F, H, J, L, N (F, H, J, L, N) | Fab antibody fragment, light chain (10D10) | polymer | 212 | 23339.7 | 5 | Mus musculus (mouse) | ||
| 3 | G, I, K, M, O (G, I, K, M, O) | Fab antibody fragment, heavy chain (10D10) | polymer | 217 | 23638.5 | 5 | Mus musculus (mouse) | ||
| 4 | AA, DA, FA, GA, KA... (B, C, D, A, E) | CHLORIDE ION | non-polymer | 35.5 | 15 | Chemie (CL) | |||
| 5 | EA, HA, QA, R, V (C, E, A) | POTASSIUM ION | non-polymer | 39.1 | 5 | Chemie (K) | |||
| 6 | BA, IA, NA, S, W (C, D, E, A, B) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 7 | CA, JA, OA, T, X (C, D, E, A, B) | CALCIUM ION | non-polymer | 40.1 | 5 | Chemie (CA) | |||
| 8 | TA, UA, VA, WA, XA (A, B, C, D, E) | water | water | 18.0 | 10 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E: 2 - 405 (UniProt: E1C3A0)
| PDB | External Database | Details |
|---|---|---|
| Ala 76 | Ile 76 | engineered mutation |
| Ala 80 | Phe 80 | engineered mutation |
| Ala 84 | Phe 84 | engineered mutation |
| Glu 406 | - | expression tag |
| Gly 407 | - | expression tag |
| Glu 408 | - | expression tag |
| Glu 409 | - | expression tag |
| Phe 410 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 471991.5 | |
| Non-Polymers* | Number of molecules | 30 |
| Total formula weight | 1388.1 | |
| All* | Total formula weight | 473379.7 |
