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5T2N

Engineered variant of I-OnuI meganuclease targeting the Anopheles AGAP007280 gene; harbors 38 point mutations relative to wild-type I-OnuI

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)
I-OnuI_e-ag007820polymer29634192.81synthetic construct
2B
(B)
DNA (26-MER)polymer267802.01synthetic construct
3C
(C)
DNA (26-MER)polymer268176.31synthetic construct
4D, E, F, G
(A)
CALCIUM IONnon-polymer40.14Chemie (CA)
5H, I, J
(A, B, C)
waterwater18.059Chemie (HOH)
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains3
Total formula weight50171.1
Non-Polymers*Number of molecules4
Total formula weight160.3
All*Total formula weight50331.4
*Water molecules are not included.

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PDB entries from 2025-06-18

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