5T2N
Engineered variant of I-OnuI meganuclease targeting the Anopheles AGAP007280 gene; harbors 38 point mutations relative to wild-type I-OnuI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 108 |
| Detector technology | PIXEL |
| Collection date | 2016-05-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.084 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.834, 67.421, 166.883 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.908 - 2.079 |
| R-factor | 0.2226 |
| Rwork | 0.220 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qqy |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.697 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.150 |
| High resolution limit [Å] | 2.080 | 4.480 | 2.080 |
| Rmerge | 0.094 | 0.072 | 0.793 |
| Rmeas | 0.098 | 0.076 | 0.839 |
| Rpim | 0.028 | 0.022 | 0.266 |
| Total number of observations | 339574 | ||
| Number of reflections | 28554 | ||
| <I/σ(I)> | 10.1 | ||
| Completeness [%] | 99.6 | 99.4 | 99 |
| Redundancy | 11.9 | 11.8 | 8.9 |
| CC(1/2) | 0.995 | 0.883 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.05M ammonium sulfate, 0.05M BIS-TRIS pH 6.5, 35% pentaerythritol ethoxylate (15/4 EO/OH) |
