5T04

STRUCTURE OF CONSTITUTIVELY ACTIVE NEUROTENSIN RECEPTOR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Neurotensin receptor type 1,Endolysin,Neurotensin receptor type 1	polymer	513	57613.4	1	UniProt (P20789) UniProt (P00720) Pfam (PF00001) Pfam (PF00959) In PDB	Rattus norvegicus (Rat)	NTR1,High-affinity levocabastine-insensitive neurotensin receptor,NTRH,Lysis protein,Lysozyme,Muramidase,NTR1,High-affinity levocabastine-insensitive neurotensin receptor,NTRH
2	B	ARG-ARG-PRO-TYR-ILE-LEU	polymer	6	819.0	1	UniProt (by SIFTS) (P20068) In PDB	Rattus norvegicus
3	A	3,3',3''-phosphanetriyltripropanoic acid	non-polymer		250.2	1	Chemie (TCE)
4	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)

Sequence modifications

A: 43 - 268 (UniProt: P20789)

PDB	External Database	Details
Asp 33	-	expression tag
Tyr 34	-	expression tag
Lys 35	-	expression tag
Asp 36	-	expression tag
Asp 37	-	expression tag
Asp 38	-	expression tag
Asp 39	-	expression tag
Ala 40	-	expression tag
Thr 41	-	expression tag
Ser 42	-	expression tag
Leu 86	Ala 86	engineered mutation
Ala 215	Gly 215	engineered mutation

A: 1002 - 1161 (UniProt: P00720)

PDB	External Database	Details
Gly 1012	Arg 12	engineered mutation
Thr 1054	Cys 54	engineered mutation
Ala 1097	Cys 97	engineered mutation
Asn 1122	Gln 122	engineered mutation
Asn 1123	Gln 123	engineered mutation
Arg 1137	Ile 137	engineered mutation
Gly 1162	-	linker
Ser 1163	-	linker
Gly 1164	-	linker
Ser 1165	-	linker

A: 297 - 396 (UniProt: P20789)

PDB	External Database	Details
Ala 358	Phe 358	engineered mutation
Ala 360	Val 360	engineered mutation
Ala 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag
Gly 408	-	expression tag
Gly 409	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58432.5
Non-Polymers*	Number of molecules	5
	Total formula weight	660.6
All*	Total formula weight	59093.1

*Water molecules are not included.