5T04
STRUCTURE OF CONSTITUTIVELY ACTIVE NEUROTENSIN RECEPTOR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2015-06-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 104.190, 75.710, 83.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.141 - 3.300 |
R-factor | 0.2555 |
Rwork | 0.253 |
R-free | 0.28330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xee |
RMSD bond length | 0.002 |
RMSD bond angle | 0.494 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.141 | 3.560 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.180 | 0.720 |
Number of reflections | 10042 | |
<I/σ(I)> | 8.3 | 1.6 |
Completeness [%] | 97.3 | 88.3 |
Redundancy | 7.1 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 13-16% (v/v) PEG 400, 80 mM TrisHCl pH 8.5-9.0, 1.9 mM TCEP, 68-91 mM lithium acetate, 0.9 mM Neurotensin | |
1 | LIPIDIC CUBIC PHASE | 293 | 13-16% (v/v) PEG 400, 80 mM TrisHCl pH 8.5-9.0, 1.9 mM TCEP, 68-91 mM lithium acetate, 0.9 mM Neurotensin |